Glossary entry (derived from question below)
Dec 7, 2005 22:55
18 yrs ago
9 viewers *
Spanish term
ADN templado
Spanish to English
Science
Biology (-tech,-chem,micro-)
Biotechnology studies
¿¿En este caso se refiere a la temperatura del DNA, o a algún procedimiento especial??
La mezcla de reacción usada para la PCR contenía: 100 ng de ******ADN templado****** de P. capsici, 0.2 mM de cada iniciador, 2.5 mM de cada dNTP, 15 mM MgCl2, 100 mM Tris-HCl (pH 8), 500 mM KCl, 3 unidades de Taq polimerasa (Boehringer, Mannheim, Indianapolis, 1N), en un volumen final de 50 l (Medina, 2002).
La mezcla de reacción usada para la PCR contenía: 100 ng de ******ADN templado****** de P. capsici, 0.2 mM de cada iniciador, 2.5 mM de cada dNTP, 15 mM MgCl2, 100 mM Tris-HCl (pH 8), 500 mM KCl, 3 unidades de Taq polimerasa (Boehringer, Mannheim, Indianapolis, 1N), en un volumen final de 50 l (Medina, 2002).
Proposed translations
(English)
| 5 +3 | template DNA |
PaulinaRich
|
| 5 | templateD DNA |
celiacp
|
| 3 | room-temperature DNA |
Robert Forstag
|
Proposed translations
+3
55 mins
PaulinaRich
United States
United States
Native in Spanish
Specializes in field
United States
Related KudoZ points
:
4
Selected
template DNA
Could it be refering to a template strand of DNA to be used in a PCR (polimerase chain reaction)?
I used to work in a lab doing PCRs only and we used DNA templates to replicate it.
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Note added at 59 mins (2005-12-07 23:54:55 GMT)
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Protocol for PCR using Taq DNA PolymeraseA control reaction, omiting template DNA, should always be performed, ... The number of PCR cycles depends on the amount of ***template DNA*** in the reaction mix ...
www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm
Revista chilena de infectolog - <I>Biolog molecular en ... - [ Translate this page ]
Cada hebra de ***ADN (templado)*** sirve como molde para la amplificación y sólo ...
El ADN doble hebra formado (templado: partidor) definirá el sitio de acción ...
http://www.scielo.cl/scielo.php?pid=S0716-10182002000100003&...
Preparation of ***Template for DNA Sequencing***The quality of the DNA template is one of the most critical factors in ... If you have enough template DNA, always determine its concentration by UV ...
seqcore.brcf.med.umich.edu/doc/dnaseq/dnaprep.html
I used to work in a lab doing PCRs only and we used DNA templates to replicate it.
--------------------------------------------------
Note added at 59 mins (2005-12-07 23:54:55 GMT)
--------------------------------------------------
Protocol for PCR using Taq DNA PolymeraseA control reaction, omiting template DNA, should always be performed, ... The number of PCR cycles depends on the amount of ***template DNA*** in the reaction mix ...
www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm
Revista chilena de infectolog - <I>Biolog molecular en ... - [ Translate this page ]
Cada hebra de ***ADN (templado)*** sirve como molde para la amplificación y sólo ...
El ADN doble hebra formado (templado: partidor) definirá el sitio de acción ...
http://www.scielo.cl/scielo.php?pid=S0716-10182002000100003&...
Preparation of ***Template for DNA Sequencing***The quality of the DNA template is one of the most critical factors in ... If you have enough template DNA, always determine its concentration by UV ...
seqcore.brcf.med.umich.edu/doc/dnaseq/dnaprep.html
4 KudoZ points awarded for this answer.
Comment: "¡Muchas gracias, Paulina y a todos por su ayuda!"
2 mins
Robert Forstag
United States
United States
Native in English
United States
Translating words, preserving meaning
Native in: English
Works in: Spanish to English, English
room-temperature DNA
The term refers to the temperature of the DNA being used.
Suerte.
Suerte.
11 hrs
templateD DNA
MCDB 2150 -- Lecture 3 - [ Traduzca esta página ]
Templated DNA synthesis: After the RNA primer has reached an adequate length,
DNA polymerase III begins synthesis of DNA, which proceeds to completion in ...
www.colorado.edu/MCDB/MCDB2150Fall/notes00/L0003.html - 22k - En caché - Páginas similares
Ellenberger Lab Research - [ Traduzca esta página ]
We have a long-standing interest in the mechanism of templated DNA synthesis by
DNA polymerases. We are investigating bacteriophage T7 DNA replication ...
biochem.wustl.edu/~tome/research.htm - 15k - 6 Dic 2005 - En caché - Páginas similares
Ahí encontrarás la explicación de lo que es el ADN templado. Suerte en tu traducción!
Templated DNA synthesis: After the RNA primer has reached an adequate length,
DNA polymerase III begins synthesis of DNA, which proceeds to completion in ...
www.colorado.edu/MCDB/MCDB2150Fall/notes00/L0003.html - 22k - En caché - Páginas similares
Ellenberger Lab Research - [ Traduzca esta página ]
We have a long-standing interest in the mechanism of templated DNA synthesis by
DNA polymerases. We are investigating bacteriophage T7 DNA replication ...
biochem.wustl.edu/~tome/research.htm - 15k - 6 Dic 2005 - En caché - Páginas similares
Ahí encontrarás la explicación de lo que es el ADN templado. Suerte en tu traducción!
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