Glossary entry

English term or phrase:

Isocratic

English answer:

constant mobile phase composition

Added to glossary by Lamprini Kosma
Aug 23, 2004 08:35
19 yrs ago
3 viewers *
English term

Isocratic

English Tech/Engineering Chemistry; Chem Sci/Eng
Context: Analytical HPLC was carried out using one of the following methods:

Method A: Waters pump (600), Waters differential refractometer (410), RI detector settings: scale factor = 20, sensitivity = 2, oven temperature = 35 oC. Phenomenex Luna C18(2), 4.6 x 150 mm, 5 µm; Isocratic MeCN/H2O (30/70); 1 mL/min; injection volume: 20 µL.
What dose " Isocratic" in the second line from the bottom. Does it mean equal?

Discussion

Non-ProZ.com Aug 24, 2004:
Sorry! I had chosen a wrong answer in grading in hurry. From the angle of chemistry, Lamprini Kosma's answer is more appropriate. But thank you doctor Powers for efforts and help!

Responses

+8
9 mins
Selected

constant mobile phase composition

I hope you'll find the following link useful:

"Isocratic

A constant composition mobile phase used in liquid chromatography."

http://www.waters.com/watersdivision/images/aboutus/hplcglos...
Peer comment(s):

agree n/a (X) : Exactly, distinct from 'gradient HPLC'
1 min
Thanks :-)
agree Saleh Chowdhury, Ph.D.
5 mins
Thanks :-)
agree Elena Petelos : :-))
28 mins
Thanks! :-)
agree Cilian O'Tuama
44 mins
Thanks, Cilian! :-)
agree Andrés Martínez : Absolutely, in this context
44 mins
Thanks :-)
agree Jörgen Slet
45 mins
Thanks :-)
agree Ramesh Madhavan
1 hr
Thanks :-)
agree achisholm : spot on
3 hrs
Thanks, Alexander! :-)
Something went wrong...
4 KudoZ points awarded for this answer. Comment: "Thank You!"
+4
4 mins

equal

isocracy
n. equal rule; possession of equal power by all. isocrat, n. isocratic, a.


© From the Hutchinson Encyclopaedia.
Helicon Publishing LTD 2000.
All rights reserved.


Dictionary search Search for:




Syria Flag The stars are said to represent Syria and Iraq. Red, white, black, and green are the pan-Arab colours. Effective date 29 March 1980. >>

Mike :)

--------------------------------------------------
Note added at 8 mins (2004-08-23 08:44:06 GMT)
--------------------------------------------------

It means \"equal\" in the sense that they stay the same. Look at the following:

Isocratic Elution
Refers to an HPLC separation where the mobile phase composition stays the same during the entire analysis. Isocratic separations are the opposite of GRADIENT ELUTION separations.


Upchurch Scientific - Glossary
... Gradient Elution An HPLC procedure where the composition of the mobile phase changes
during the separation; opposite of ISOCRATIC ELUTION where the mobile ...
www.upchurch.com/TechInfo/glossary.asp - 101k - Cached - Similar pages

Mike :)
Peer comment(s):

agree Vicky Papaprodromou
2 mins
Thank you, Vicky - Mike :)
agree Saleh Chowdhury, Ph.D.
10 mins
Thank you, Saleh - Mike :)
agree eldira
29 mins
agree Jörgen Slet
51 mins
Something went wrong...
Term search
  • All of ProZ.com
  • Term search
  • Jobs
  • Forums
  • Multiple search