English to Ukrainian Medical Medical (general)

Each organism suspension was prepared to achieve an approximate concentration of 1 x 108 colony-forming units (CFU)/mL. Each of the five tubes containing 10 mL of sample was inoculated with 0.05 mL of its corresponding challenge organism, resulting in an initial concentration from between 1 x 105 CFU/mL and 1 x 106 CFU/mL of the test preparation. The initial population of viable organisms in each test preparation was calculated based on the concentration of the organisms in each of the standardized inocula by the Pour Plate Method. This method is a common microbiological technique to count the number of organisms in a test sample. The test sample is diluted with sterile saline and pipetted onto a sterile Petri dish. Then melted agar (the nutrient media) is poured into the Petri dish and mixed with the sample and incubated at a standardized temperature and time. This method yields colonies that form throughout the agar - not just on the surface.